Bioactive Nitrosylated and Nitrated N-(2-hydroxyphenyl)acetamides and Derived Oligomers: An Alternative Pathway to 2-Amidophenol-Derived Phytotoxic Metabolites

Incubation of Aminobacter aminovorans, Paenibacillus polymyxa, and Arthrobacter MPI764 with the microbial 2-benzoxazolinone (BOA)-degradation-product 2-acetamido-phenol, produced from 2-aminophenol, led to the recently identified N-(2-hydroxy-5-nitrophenyl) acetamide, to the hitherto unknown N-(2-hydroxy-5-nitrosophenyl)acetamide, and to N-(2-hydroxy-3-nitrophenyl)acetamide. As an alternative to the formation of phenoxazinone derived from aminophenol, dimers- and trimers-transformation products have been found. Identification of the compounds was carried out by LC/HRMS and MS/MS and, for the new structure N-(2-hydroxy-5-nitrosophenyl)acetamide, additionally by 1D- and 2D-NMR. Incubation of microorganisms, such as the soil bacteria Pseudomonas laurentiana, Arthrobacter MPI763, the yeast Papiliotrema baii and Pantoea ananatis, and the plants Brassica oleracea var. gongylodes L. (kohlrabi) and Arabidopsis thaliana Col-0, with N-(2-hydroxy-5-nitrophenyl) acetamide, led to its glucoside derivative as a prominent detoxification product; in the case of Pantoea ananatis, this was together with the corresponding glucoside succinic acid ester. In contrast, Actinomucor elegans consortium synthesized 2-acetamido-4-nitrophenyl sulfate. 1 mM bioactive N-(2-hydroxy-5-nitrophenyl) acetamide elicits alterations in the Arabidopsis thaliana expression profile of several genes. The most responsive upregulated gene was pathogen-inducible terpene synthase TPS04. The bioactivity of the compound is rapidly annihilated by glucosylation

Allelopathic cover crop of rye for integrated weed control in sustainable agroecosystems

The allelopathic potential of rye ( Secale cereale L.) is mainly due to phytotoxic benzoxazinones, compounds that are produced and accumulated in young tissues to different degrees depending on cultivar and environmental influences. Living rye plants exude low levels of benzoxazinones, while cover crop residues can release from 12 to 20 kg ha –1 . This paper summarizes the results obtained from several experiments performed in both controlled and field environments, in which rye was used as a cover crop to control summer weeds in a following maize crop. Significant differences in benzoxazinoid content were detected between rye cultivars. In controlled environments, rye mulches significantly reduced germination of some broadleaf weeds. Germination and seedling growth of Amaranthus retroflexus and Portulaca oleracea were particularly affected by the application of rye mulches, while Chenopodium album was hardly influenced and Abutilon theophrasti was advantaged by the presence of the mulch. With reference to the influence of agronomic factors on the production of benzoxazinoids, nitrogen fertilization increased the content of allelochemicals, although proportionally less than dry matter. The field trial established on no-till maize confirmed the significant weed suppressiveness of rye mulch, both for grass and broadleaf weeds. A significant positive interaction between nitrogen (N) fertilization and notillage resulting in the suppression of broadleaf weeds was observed. The different behavior of the weeds in the presence of allelochemicals was explained in terms of differential uptake and translocation capabilities. The four summer weeds tested were able to grow in the presence of low amounts of benzoxazolin-2(3H)-one (BOA), between 0.3 and 20 mmol g –1 fresh weight. Although there were considerable differences in their sensitivity to higher BOA concentrations, P. oleracea , A. retroflexus , and Ch. album represented a group of species with a consistent absorption capability. The insensitivity of A . theophrasti to BOA was due to reduced accumulation in seedlings. Overall, results confirm that the use of a rye cover crop in a suitable crop rotation represents a sustainable weed management practice permitting a reduction in the amount of herbicides used in agroecosystems, thus limiting the environmental risks of intensive agriculture

'Transformations towards sustainability':Emerging approaches, critical reflections, and a research agenda

Over the last two decades researchers have come to understand much about the global challenges confronting human society (e.g. climate change; biodiversity loss; water, energy and food insecurity; poverty and widening social inequality). However, the extent to which research and policy efforts are succeeding in steering human societies towards more sustainable and just futures is unclear. Attention is increasingly turning towards better understanding how to navigate processes of social and institutional transformation to bring about more desirable trajectories of change in various sectors of human society. A major knowledge gap concerns understanding how transformations towards sustainability are conceptualised, understood and analysed. Limited existing scholarship on this topic is fragmented, sometimes overly deterministic, and weak in its capacity to critically analyse transformation processes which are inherently political and contested. This paper aims to advance understanding of transformations towards sustainability, recognising it as both a normative and an analytical concept. We firstly review existing concepts of transformation in global environmental change literature, and the role of governance in relation to it. We then propose a framework for understanding and critically analysing transformations towards sustainability based on the existing ‘Earth System Governance’ framework (Biermann et al., 2009). We then outline a research agenda, and argue that transdisciplinary research approaches and a key role for early career researchers are vital for pursuing this agenda. Finally, we argue that critical reflexivity among global environmental change scholars, both individually and collectively, will be important for developing innovative research on transformations towards sustainability to meaningfully contribute to policy and action over time

Abutilon theophrasti’s Resilience against Allelochemical-Based Weed Management in Sustainable Agriculture – Due to Collection of Highly Advantageous Microorganisms?

Abutilon theophrasti Medik. (velvetleaf) is a problematic annual weed in field crops which has invaded many temperate parts of the world. Since the loss of crop yields can be extensive, approaches to manage the weed include not only conventional methods, but also biological methods, for instance by microorganisms releasing phytotoxins and plant-derived allelochemicals. Additionally, benzoxazinoid-rich rye mulches effective in managing common weeds like Amaranthus retroflexus L. have been tested for this purpose. However, recent methods for biological control are still unreliable in terms of intensity and duration. Rye mulches were also ineffective in managing velvetleaf. In this review, we present the attempts to reduce velvetleaf infestation by biological methods and discuss possible reasons for the failure. The resilience of velvetleaf may be due to the extraordinary capacity of the plant to collect, for its own survival, the most suitable microorganisms from a given farming site, genetic and epigenetic adaptations, and a high stress memory. Such properties may have developed together with other advantageous abilities during selection by humans when the plant was used as a crop. Rewilding could be responsible for improving the microbiomes of A. theophrasti

Exploring the governance and politics of transformations towards sustainability

AbstractThe notion of ‘transformations towards sustainability’ takes an increasingly central position in global sustainability research and policy discourse in recent years. Governance and politics are central to understanding and analysing transformations towards sustainability. However, despite receiving growing attention in recent years, the governance and politics aspects of transformations remain arguably under-developed in the global sustainability literature. A variety of conceptual approaches have been developed to understand and analyse societal transition or transformation processes, including: socio-technical transitions, social-ecological systems, sustainability pathways, and transformative adaptation. This paper critically surveys these four approaches, and reflects on them through the lens of the Earth System Governance framework (Biermann et al., 2009). This contributes to appreciating existing insights on transformations, and to identifying key research challenges and opportunities. Overall, the paper brings together diverse perspectives, that have so far remained largely fragmented, in order to strengthen the foundation for future research on transformations towards sustainability

Benzoxazinoids in wheat allelopathy – From discovery to application for sustainable weed management

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGAllelopathic activity of wheat (Triticum aestivum L.) has previously been associated with the production of phenolic acids and flavonoids (PAF), benzoxazinones (BXZs) and phenoxazinones (PXZs). The biosynthesis of BXZs is closely regulated during cereal growth, with accumulation highest in young tissues with variation associated with genotype and environmental conditions. This review is focused on BXZ metabolites and their impact on germination, seedling growth and physiological, biochemical, transcriptional and proteome traits of surrounding plants and weeds. The major pathways employed by plants for benzoxazinoid detoxification involve hydroxylation and glucosylation and polymerisation of intermediates in these pathways. Allelochemicals from various wheat genotypes have been shown to inhibit the growth of selected weed species, including Bromus japonicus, Chenopodium album, Portulaca oleracea, Avena fatua and Lolium rigidum. Wheat allelopathy is potentially exploited from the standpoint of crop mulches, incorporation of crop residues, tissue disruption, intercropping with allelopathic cultivars and application of aqueous wheat extracts. BXZs have been shown to suppress the growth and development of certain agricultural pests, including insects, fungal pathogens, and weeds. Many native plants, fungi and insect herbivores inherently possess varying tolerance levels towards BXZs. However, other BXZ- susceptible species are adversely impacted by elevated BXZ levels in crop plants. Thus, considerations for the selection and breeding of wheat genotypes possessing enhanced defensive ability via elevated BXZ contents are discussed. Here, these objectives are reconsidered with a focus on co-evolutionary aspects and their potential impacts on biodiversity in the agroecosystems under study. For future breeding efforts to be successful, it is important to take such potential adverse environmental impacts into account, in combination with an increased focus on enhancing beneficial allelopathic effects within agricultural systems

Implications of Below-Ground Allelopathic Interactions of Camelina sativa and Microorganisms for Phosphate Availability and Habitat Maintenance

Toxic breakdown products of young Camelina sativa (L.) Crantz, glucosinolates can eliminate microorganisms in the soil. Since microorganisms are essential for phosphate cycling, only insensitive microorganisms with phosphate-solubilizing activity can improve C. sativa’s phosphate supply. In this study, 33P-labeled phosphate, inductively coupled plasma mass spectrometry and pot experiments unveiled that not only Trichoderma viride and Pseudomonas laurentiana used as phosphate-solubilizing inoculants, but also intrinsic soil microorganisms, including Penicillium aurantiogriseum, and the assemblies of root-colonizing microorganisms solubilized as well phosphate from apatite, trigger off competitive behavior between the organisms. Driving factors in the competitiveness are plant and microbial secondary metabolites, while glucosinolates of Camelina and their breakdown products are regarded as key compounds that inhibit the pathogen P. aurantiogriseum, but also seem to impede root colonization of T. viride. On the other hand, fungal diketopiperazine combined with glucosinolates is fatal to Camelina. The results may contribute to explain the contradictory effects of phosphate-solubilizing microorganisms when used as biofertilizers. Further studies will elucidate impacts of released secondary metabolites on coexisting microorganisms and plants under different environmental conditions

Asteroids and Comets

Asteroids and comets are remnants from the era of Solar System formation over 4.5 billion years ago, and therefore allow us to address two fundamental questions in astronomy: what was the nature of our protoplanetary disk, and how did the process of planetary accretion occur? The objects we see today have suffered many geophysically-relevant processes in the intervening eons that have altered their surfaces, interiors, and compositions. In this chapter we review our understanding of the origins and evolution of these bodies, discuss the wealth of science returned from spacecraft missions, and motivate important questions to be addressed in the future.Comment: 84 pages, 27 figures. To be published in Treatise on Geophysics, 2nd edition (G. Schubert, Editor-in-Chief), Volume 10 (T. Spohn, Editor